Our platform can screen up to 200,000 compounds in 15 days using various GPCR assays (Calcium, cAMP, IP-One, ERK1/2, GTPg35S binding) for various modes of action (orthosteric and/or allosteric, agonist and/or antagonist). Our programs include assay development and validation, Client’s library formatting and handling, HTS, confirmation of positives, dose response curves of confirmed actives and selectivity determination.
Hamamatsu FDSS-6000 for Calcium Luminescence
BMG Rubystar for HTRFTM (cAMP IP-One, enzymatic)
TopCount HTS for GTPg35S and radioligand binding
TECAN Infinite200Pro for BRET, fluorescence intensity and polarization assays
Minitrack for library reformatting and assay plates replication
Janus for cherry picking and dose-response curves generation